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Product Info Summary
| SKU: | A00204-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ran Antibody Picoband™
SKU/Catalog Number
A00204-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ran Antibody Picoband™ catalog # A00204-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ran Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00204-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Ran recombinant protein (Position: A2-L216). Human Ran shares 100% amino acid (aa) sequence identity with both mouse and rat Ran.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00204-1 is reactive to RAN in Human, Mouse, Rat
Applications
A00204-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
24 kDa
Calculated molecular weight
24.423kDa
Background of RAN
RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat,
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
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Figure 5. IHC analysis of Ran using anti-Ran antibody (A00204-1).
Ran was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ran Antibody (A00204-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of Ran using anti-Ran antibody (A00204-1).
Ran was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ran Antibody (A00204-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Ran using anti-Ran antibody (A00204-1).
Ran was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ran Antibody (A00204-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of Ran using anti-Ran antibody (A00204-1).
Ran was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ran Antibody (A00204-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Ran using anti-Ran antibody (A00204-1).
Ran was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ran Antibody (A00204-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 1. Western blot analysis of Ran using anti-Ran antibody (A00204-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: rat testis tissue lysate,
Lane 3: rat thymus tissue lysate,
Lane 4: mouse brain tissue lysate,
Lane 5: mouse testis tissue lysate,
Lane 6: mouse thymus tissue lysate,
Lane 7: human A549 whole cell lysate,
Lane 8: human 22RV1 whole cell lysate,
Lane 9: human Hela whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ran antigen affinity purified polyclonal antibody (Catalog # A00204-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ran at approximately 24KD. The expected band size for Ran is at 24KD.
Click image to see more details
Figure 7. IF analysis of Ran using anti-Ran antibody (A00204-1)
Ran was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Ran Antibody (A00204-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 8. Flow Cytometry analysis of A431 cells using anti-Ran antibody (A00204-1).
Overlay histogram showing A431 cells stained with A00204-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ran Antibody (A00204-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 9. Flow Cytometry analysis of U937 cells using anti-Ran antibody (A00204-1).
Overlay histogram showing U937 cells stained with A00204-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ran Antibody (A00204-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For RAN (Source: Uniprot.org, NCBI)
Gene Name
RAN
Full Name
GTP-binding nuclear protein Ran
Weight
24.423kDa
Superfamily
small GTPase superfamily
Alternative Names
Androgen receptor-associated protein 24; ARA24Gsp1; GTPase Ran; GTP-binding nuclear protein Ran; guanosine triphosphatase Ran; member RAS oncogene family; OK/SW-cl.81; RAN, member RAS oncogene family; RanGTPase; Ras-like protein TC4; Ras-related nuclear protein; TC4 RAN ARA24, Gsp1, TC4 RAN, member RAS oncogene family GTP-binding nuclear protein Ran|GTPase Ran|RanGTPase|androgen receptor-associated protein 24|guanosine triphosphatase Ran|member RAS oncogene family|ras-like protein TC4|ras-related nuclear protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAN, check out the RAN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ran Antibody Picoband™ (A00204-1)
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No publications found for A00204-1
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-Ran Antibody Picoband™
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical cord blood using anti-Ran antibody A00204-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-23
Question
Here is the WB image, lot number and protocol we used for umbilical cord blood using anti-Ran antibody A00204-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-18
Question
I see that the anti-Ran antibody A00204-1 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-04
Question
I have a question about product A00204-1, anti-Ran antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00204-1 anti-Ran antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-02
Question
Will anti-Ran antibody A00204-1 work for ICC with umbilical cord blood?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
According to the expression profile of umbilical cord blood, RAN is highly expressed in umbilical cord blood. So, it is likely that anti-Ran antibody A00204-1 will work for ICC with umbilical cord blood.
Boster Scientific Support
Answered: 2019-08-13
Question
We are currently using anti-Ran antibody A00204-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
N. Anderson
Verified customer
Asked: 2019-05-14
Answer
The anti-Ran antibody (A00204-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-14
Question
Is this A00204-1 anti-Ran antibody reactive to the isotypes of RAN?
Verified Customer
Verified customer
Asked: 2018-04-23
Answer
The immunogen of A00204-1 anti-Ran antibody is E. coli-derived human Ran recombinant protein (Position: A2-L216). Human Ran shares 100% amino acid (aa) sequence identity with both mouse and rat Ran. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-23
Question
I am interested in to test anti-Ran antibody A00204-1 on human umbilical cord blood for research purposes, then I may be interested in using anti-Ran antibody A00204-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
The products we sell, including anti-Ran antibody A00204-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-01
Question
Do you have a BSA free version of anti-Ran antibody A00204-1 available?
J. Thomas
Verified customer
Asked: 2017-10-09
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Ran antibody A00204-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-09
Question
Does anti-Ran antibody A00204-1 work on feline IHC-P with lymph?
Verified Customer
Verified customer
Asked: 2017-09-04
Answer
Our lab technicians have not tested anti-Ran antibody A00204-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Ran antibody A00204-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline lymph in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-04
Question
We have observed staining in mouse melanoma. Are there any suggestions? Is anti-Ran antibody supposed to stain melanoma positively?
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
From literature melanoma does express RAN. From Uniprot.org, RAN is expressed in testis, teratocarcinoma, brain, umbilical cord blood, hippocampus, lymph, ovary, skin uterus, platelet, brain cajal-retzius cell, colon adenocarcinoma, cervix carcinoma, lymphoblast, melanoma, erythroleukemia, liver, among other tissues. Regarding which tissues have RAN expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8421051, 10400640
Cervix carcinoma, Pubmed ID: 1961752
Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymph, Ovary, Skin, and Uterus, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Teratocarcinoma, Pubmed ID: 2108320
Umbilical cord blood, Pubmed ID: 11042152
Boster Scientific Support
Answered: 2017-05-22
Question
I am interested in using your anti-Ran antibody for mitotic cell cycle studies. Has this antibody been tested with western blotting on mammary cancer tissue? We would like to see some validation images before ordering.
S. Miller
Verified customer
Asked: 2016-11-15
Answer
Thanks for your inquiry. This A00204-1 anti-Ran antibody is tested on rat thymus tissue, brain tissue, tissue lysate, testis tissue, mouse thymus tissue, human hela, a549 whole cell lysate, 22rv1 whole cell lysate, hela whole cell lysate, intestinal cancer tissue, lung cancer tissue, mammary cancer tissue, small intestine tissue, a431 cells, u937 cells. It is guaranteed to work for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-11-15
Question
Our lab were happy with the WB result of your anti-Ran antibody. However we have seen positive staining in skin uterus nucleus using this antibody. Is that expected? Could you tell me where is RAN supposed to be expressed?
J. Johnson
Verified customer
Asked: 2016-09-08
Answer
From what I have seen in literature, skin uterus does express RAN. Generally RAN expresses in nucleus. Regarding which tissues have RAN expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8421051, 10400640
Cervix carcinoma, Pubmed ID: 1961752
Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymph, Ovary, Skin, and Uterus, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Teratocarcinoma, Pubmed ID: 2108320
Umbilical cord blood, Pubmed ID: 11042152
Boster Scientific Support
Answered: 2016-09-08
Question
I was wanting to use your anti-Ran antibody for ICC for human umbilical cord blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human umbilical cord blood identification?
T. Baker
Verified customer
Asked: 2016-03-31
Answer
As indicated on the product datasheet, A00204-1 anti-Ran antibody has been tested for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human umbilical cord blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-31
Question
Is a blocking peptide available for product anti-Ran antibody (A00204-1)?
D. Jones
Verified customer
Asked: 2015-03-13
Answer
We do provide the blocking peptide for product anti-Ran antibody (A00204-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-03-13
Question
Does A00204-1 anti-Ran antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Krishna
Verified customer
Asked: 2013-05-13
Answer
You can see on the product datasheet, A00204-1 anti-Ran antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-05-13
Question
We ordered your anti-Ran antibody for IHC-F on ovary a few months ago. I am using rat, and I plan to use the antibody for IHC-P next. I am looking for examining ovary as well as skin uterus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
F. Mangal
Verified customer
Asked: 2013-04-22
Answer
I have checked the website and datasheets of our anti-Ran antibody and I see that A00204-1 has been validated on rat in both IHC-F and IHC-P. Thus A00204-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-04-22
